FIG 3.

Mce4A and Mce4E protein levels are reduced in the absence of individual Mce4, Mam4, OmamA, and OmamB proteins. Protein levels of Mce4A (A) and Mce4E (B) were assessed in WT, mutant, and complemented M. smegmatis strains via immunoblotting. The arrow indicates the Mce4A band, to distinguish it from the higher species cross-reacting band. Mutant strains contain empty vectors (EV) as indicated. Protein levels of GroEL were assessed to ensure equal protein loading across strains. Results are representative of at least three independent replicates.