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. 2021 May 1;40:151. doi: 10.1186/s13046-021-01957-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — CTSL1 was upregulated in ovarian cancer. a Single-cell RNA sequencing (scRNA-seq) analysis of ovarian cancer. The heatmap shows cell marker (EPCAM for epithelial cells, CD14 for monocytes/macrophages, CD4 for CD4⁺ T cells) expression patterns in CTSL1⁺ cells. b scRNA-seq in EPCAM⁺ cells from three ovarian cancer para-tumor tissues (used as control), ovarian cancer tissue, and the matched peritoneal metastasis tissues. c Quantification of CTSL1 expression in EPCAM⁺ cells from benign ovarian cyst, ovarian cancer, and peritoneal metastasis tissues with different CTSL1 staining. e and f Quantification of CTSL1 IHC score in different tissues. g Overall survival of ovarian cancer patients with low/high CTSL1 IHC score. h Immunoblotting of CTSL1 in different tissues. i ELISA of CTSL1 in serum and ascites of ovarian cancer patients