Fig. 2.

Reovirus-activated NK cells exhibit enhanced ADCC-mediated killing of colorectal cancer cells in combination with cetuximab. NK cells, as effectors, were activated with 10 MOI reovirus or UV-inactivated reovirus at 37℃ for 12 h. a DLD-1, or b Caco-2 and HT29 cells were used as target cells. Cetuximab and isotype control IgG1 were applied at 1 μg/mL and cytotoxic activity was measured by CCK-8 assay at an E:T = 5:1 ratio after 4 h of co-culture. *p < 0.05, **p < 0.01, ***p < 0.001. Data represent means ± SD from at least 3 independent experiments. c, d Levels of perforin secretion from reovirus-activated NK cells against DLD-1 or HT29 combined with cetuximab were determined by ELISA