| Time | B % | Flow |
|---|---|---|
| 0.0 | 3.0 | 0.30 μl/min |
| 2.5 | 3.0 | 0.30 μl/min |
| 4.0 | 8.0 | 0.30 μl/min |
| 52.5 | 26.0 | 0.30 μl/min |
| 60.0 | 35.0 | 0.30 μl/min |
| 62.0 | 70.0 | 0.30 μl/min |
| 67.0 | 70.0 | 0.30 μl/min |
| 70.0 | 3.0 | 0.30 μl/min |
| 92.0 | 3.0 | 0.30 μl/min |
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