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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: Nat Protoc. 2020 Dec 21;16(2):1034–1061. doi: 10.1038/s41596-020-00448-3

Table 1.

Recommendations on protocol modifications for selected model organisms

Organism name Amount of starting material Genome size Method variant Needed modifications
Homo sapiens 107 cells 3.1 Gb human None
Mus musculus 107 cells 2.6 Gb human None
Xenopus laevis 107 cells 3.1 Gb human None
Arabidopsis thaliana 2.5×108 cells 136 Mb human After Step 1A(viii) use cellulase-macerozyme solution53 to create protoplasts, then proceed to Step 1A(x)
Drosophila melanogaster 2.5×108 cells 139.5 Mb human None
Saccharomyces cerevisiae 2.5×109 cells 12 Mb yeast None
Schizosaccharomyces pombe 2.5×109 cells 14 Mb yeast None
Escherichia coli 7.5×109 cells 4.6 Mb yeast In Step 1A(ix) use lysozyme instead of lyticase (the exact reaction conditions need to be established, start with amount of enzyme and time of treatment used in your laboratory for standard applications)