| Component | Amount | Final Concentration |
|---|---|---|
| 10x Blunting buffer | 50 μL | 1x |
| BSA, 20 mg/mL | 2.5 μL | 100 μg/mL |
| dNTPs 1mM | 15 μL | 30 μM |
| Blunting Enzyme mix | 6 μL | |
| Water | 426.5 μL | |
| Total | 500 μL |
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