FIGURE 2.
MOF interacts with ERα to be involved in ERα protein stability. A‐C, MOF interacts with ERα. Co‐IP experiment is performed with the indicated antibodies. D, MOF is co‐localized with ERα. Immunofluorescence confocal analysis was performed with ethanol vehicle or 10−7 M E2 treatment. E, MOF has no effect on the ERα mRNA level. F, MOF overexpression increases the ERα protein level in a dose‐dependent manner. G, MOF depletion decreases the expression of ERα. H, I, MOF overexpression decreased ERα degradation. HCCLM3 and Huh7 were treated with 10 mg/mL cycloheximide (CHX) for the indicated time points, followed by western blotting analysis. J, K, MOF depletion decreases the expression of ERα. The cell lysates were subjected to western blot analysis with indicated antibodies with MG132 (5 μM) for 6 h