Skip to main content
. 2021 Mar 30;112(5):1865–1877. doi: 10.1111/cas.14836

FIGURE 3.

FIGURE 3

MOF participates in ERα acetylation to maintain ERα stability by alteration of ERα ubiquitination. A, B, MOF participates in acetylation of ERα. HCCLM3 and Huh7 cells were transfected with ERα and FLAG‐MOF (A) or shMOF (B). After incubation with TSA (5 μM) and 5 mM nicotinamide (5 μM) for 12 h, and MG132 (5 μM) for 6 h, cell lysates were subjected to IP assays with ERα antibody, western blot detected with indicated antibodies. C, MOF acetylates the K266, K268, and K299 sites of ERα. D, MG149 reduces ERα protein expression in a dose‐dependent manner. E, MG149 inhibits ERα acetylation induced by MOF. F, MG149 prevents MOF‐mediated ERα stability. G, MOF weakens the polyubiquitination of ERα in HEK293, HCCLM3, and Huh7 cells. H, Loss of function of MOF acetylase activity by FLAG‐MOF mutant (FLAG‐MOF K274R) does not affect ERα polyubiquitination. I, MOF inhibitor MG149 increases ERα polyubiquitination level. J, K, ERα mutants (K266R, K268R, K299R) carrying loss of function of ERα acetylation reverses the effect of MOF on attenuation of ERα polyubiquitination in HCCLM3 and HEK293 cells