Figure 4:

Four hours of hyperoxia causes an increase in expression of fusion proteins and mitochondrial mass which does not persist in recovery. A, Gene expression of fusion proteins (Mfn1, Mfn2, Opa1) measured by Taqman acutely (top) and with 24 hours recovery (bottom). B, Gene expression of fission proteins (Drp1, Fis1, Mtp18) measured by Taqman acutely (top) and with 24 hours recovery (bottom). C, Mitochondrial mass was determined by measuring the fluorescence signal of MitoTracker Green (MTG) using FACS at 4h and with 24 h recovery (rec). Closed bars represent air exposed cells and open bars represent cells exposed to hyperoxia for 4 hours acutely or with 24 h recovery as indicated. *p<0.05 vs Air.