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. 2021 May 3;11:11. doi: 10.1186/s13395-021-00267-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Hierarchical clustering analysis of DEGs and DEPs identified in the diaphragm and plasma in response to CMV. a Schematic of experimental design. b Heatmap of DEGs and c Heatmap of DEPs in the diaphragm among 0-day controls and 5-day CMV groups in both young and old rats. d Heatmap of DEPs in the plasma among 0-day controls and 5-day CMV groups in both young and old rats. CMV: controlled mechanical ventilation; DEGs: differentially expressed genes; DEPs: differentially expressed proteins. Y0DDia (Y5DDia) represents diaphragm sample of young rat from 0-day control (5-day CMV); Y0DPla (Y5Dpla) represents plasma sample of young rat from 0-day control (5-day CMV); O0DDia (O5DDia) represents diaphragm sample of old rat from 0-day control (5-day CMV); O0DPla (O5Dpla) represents plasma sample of old rat from 0-day control (5-day CMV)