Figure 7.

Cabozantinib treatment enhances the anti-tumor effects of T cells. (A) T cells were isolated from tumors of BALB/c mice that received vehicle or cabozantinib treatment as in Figure 2A and were cocultured with Renca cells at a ratio of E:T=2:1. The cytotoxicity of T cells was measured by RTCA (n=3 for biological replicate). (B, C) Representative plots and quantitative results of IFN-γ production by splenic T cells by ELISPOT assay. T cells were purified from splenocytes and were then stimulated with PMA (500 ng/ml) and ionomycin (10 μg/ml). IFN-γ spots were analyzed (n=3 for biological replicate). (D) Schematic diagram of the experimental design. T cells isolated from vehicle or cabozantinib treated tumor bearing BALB/c mice were adoptively transferred to NPG mice with palpable RCC tumors. (E) Tumor growth curves of NPG mice receiving T cells from different sources (n=5 per group). (F) Representative tumor images from different treatment groups. These experiments were repeated 2 times. *P<0.05, **P<0.01 by unpaired t-test.