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. 2021 Apr 19;12:658048. doi: 10.3389/fimmu.2021.658048

Figure 1.

Figure 1

B cell-intrinsic role of SHARPIN in the GC development and Ab responses. (A, B) Flow cytometry analysis of different immune cell populations, as indicated, in the spleen of B-Sharpin+/+ or B-Sharpincpdmmice immunized with NP-CGG/alum for 14 d. (C) Fluorescence imaging analysis of GCs in the spleen in mice, as in (A, B). (D) Flow cytometry analysis of plasma cells, GC B cells, and GC B cell proliferation (by BrdU incorporation) in immunized mice. (E–G) ELISPOT analyses of ASCs producing NP-specific IgM or IgG1 in the spleen and the bone marrow in mice 14 d after immunization (E) and ELISA of circulating NP7-binding and NP34-binding Ig Abs 7 and 14 d after immunization (F, G). (H, I) ELISPOT analyses of ASCs producing total IgM or IgG (H) and ELISA of circulating total Ig Abs (I) in mice, as in (E, F). Data are pooled from 2 independent experiments. (J) Flow cytometry analysis of surface IgG1 expression in GC and non-GC B cells. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; ns, not significant; t-test.