Table 1.
Real-time reverse transcription PCR assays authorized by the Italian Ministry of Health
| Assay | Specimen type | Gene target | LOD | Manufacturer |
|---|---|---|---|---|
| Bosphore Novel Coronavirus (2019-Ncov) detection kit | Nasopharyngeal swab, oropharyngeal swab, sputum, bronchoalveolar lavage | E, orflab | 25 copies/reaction | Anatolia Genetik, Turkey |
| STANDARD M nCoV Real-Time Detection Kit | Nasopharyngeal swab and throat swab, sputum | E, ORFlab | NA* | SD BIOSENSOR Inc, Korea |
| AllplexTM SARS-CoV-2 assay | Sputum, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage, throat swab | E, RdRp, N, S | 50 copies/reaction | Seegene Inc., Korea |
| QUANTY COVID-19 | Nasopharyngeal swab, oropharyngeal swab, sputum, serum | N | NA* | CLONIT SRL, Italy |
| GENEFINDER COVID-19 PLUS REALAMP KIT | Bronchoalveolar lavage fluid, throat swab, sputum | E, RdRp, N | 10 copies/reaction | OSANG HEALTHCARE Co., Korea |
| Novel Coronavirus COVID-19 (2019 nCoV) Real Time Multiplex RT PCR Kit |
Nasopharyngeal swab, oropharyngeal swab, bronchoalveolar lavage, sputum, endotracheal aspirate | E, N, ORFlab | 1×103 copies/mL | Liferiver, SHANGHAI ZI BIO-TECH CO., LTD, China |
| LabGunTM COVID-19 RT-PCR Kit | Nasopharyngeal swab, oropharyngeal swab, nasopharyngeal wash/aspirate, nasal aspirate, sputum | E, RdRp | 20 copies/µL | LabGenomics Co., Ltd, Korea |
| REALQUALITY RQ-2019-nCoV | Nasopharyngeal swab, bronchoalveolar lavage fluid, sputum | E, RdRp | NA* | AB ANALITICA s.r.l., Italy |
| COVID-19 detection kit | Nasopharyngeal swab, oropharyngeal swab, brongho-alveolar lavage | N, ORFlab | 500 copies/mL | OACP S.R.L., Italy |
*
NA, not available; COVID-19, coronavirus disease 2019; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; LOD, limit of detection.