Fig. 1.

Recombinant C-C motif chemokine ligand 11 (CCL11) stimulates in vitro myogenesis. (A) Mouse C2C12 myoblasts (MBs) were differentiated into myotubes (MTs) with 2% horse serum after exposure to the indicated concentrations of recombinant CCL11 for 4 days. MTs were stained with the anti-myosin heavy chain (MyHC) antibody while the nuclei were counterstained with 4,6-diamidino-2-phenyindole (DAPI). Quantitative results per field are presented. Scale bars, 100 μm. (B) Western blot and (C) quantitative reverse-transcription polymerase chain reaction analyses of myogenin and MyHC in C2C12 cells without or with 2% horse serum in the presence of the indicated concentrations of recombinant CCL11 for 3 days, respectively. Data are expressed as mean±standard error of mean. ^aP<0.05 vs. control or MB; ^bP<0.05 vs. CCL11 1 nM or untreated MT.