Figure EV1. Dnmt3a levels are upregulated in soleus and GA muscles after a bout of endurance exercise.

• A
  Ppargc1a was measured in various muscle types from C57BL/6J mice at rest and after a bout of low‐intensity exercise (n = 6 Soleus, n = 5 GA, n = 3 EDL Sedentary and n = 5 EDL exercise, means ± SEM, *P < 0.05, two‐tailed Student's t‐test).
• B–D
  Transcript levels of genes encoding de novo DNMTs were measured in soleus (B), GA (C), and EDL (D) from C57BL/6J mice at rest and after a bout of low‐intensity exercise (B: n = 6 Sedentary and n = 4 Exercise for Dnmt3a measurement and n = 5 for Dnmt3b measurement, C: n = 5, D: n = 3 Sedentary and n = 5 Exercise. means ± SEM, *P < 0.05, two‐tailed Student's t‐test).
• E, F
  (E) Soleus DNMT3A protein level in the nuclear fraction from (A) was measured by immunoblotting and (F) normalizing to Histone H3 using ImageJ.

Source data are available online for this figure.