Figure 2. MCK‐Dnmt3a KO mice display increased muscle damage following exercise.

• A
  The H&E staining of MCK‐KO and WT soleus muscle after a bout of low‐intensity treadmill running (top 40×, bottom 100× magnifications). Black arrow indicates centralized nuclei.
• B
  The percentage of myofibers with centralized nuclei was determined by manual counting 300 myofibers in 20× magnification. (n = 3, means ± SEM, *P < 0.05, two‐tailed Student's t‐test).
• C, D
  The analogous set of data is shown with WT and MCK‐KO GA muscles. (n = 3, means ± SEM, two‐tailed Student's t‐test. GA, gastrocnemius).
• E–G
  Hydrogen peroxide (H₂O₂) levels were measured in WT and MCK‐KO soleus (E), GA (F), and EDL (G) at rest and after a bout of exercise (n = 6 per group, means ± SEM, *P < 0.05, two‐tailed Student's t‐test and two‐way ANOVA followed by Bonferroni post hoc testing).
• H
  Serum levels of lactate were measured before and after a bout of low‐intense exercise for 50 min in WT and MCK‐KO mice (n = 16 for sedentary and n = 14 for exercise groups, means ± SEM, *P < 0.05, two‐tailed Student's t‐test and two‐way ANOVA followed by Bonferroni post hoc testing).

Source data are available online for this figure.