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. 2021 Feb 19;40(9):e106113. doi: 10.15252/embj.2020106113

Figure EV1. Reduced apparent molecular weight of VE‐cadherin proform is due to immature glycosylation.

Figure EV1

bEnd.5 cells were treated with tunicamycin or DMSO for 48 h prior to cell lysis. VE‐cadherin was precipitated from cell lysates with the antibodies C5 (mature VE‐cadherin) or VD47 (proform of VE‐cadherin). Precipitates were analyzed by Western blotting with antibodies against VE‐cadherin. Molecular weight marker is indicated in kDa.