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. 2021 Feb 8;40(9):e107015. doi: 10.15252/embj.2020107015

Figure 5. CTD shortening impairs transcription induction.

Figure 5

  1. Changes in RNA synthesis in response to 15 min of TPA treatment. MA plot showing RNA synthesis changes in TT‐seq datasets upon 15‐min treatment with TPA (200 nM) in RPB1‐52R and RPB1‐25R cells. DMSO treatment of the respective cell line was used as a control, and the data were normalized using library size normalization. 14,636 expressed genes annotated in RefSeq were analyzed. Differentially expressed genes are in red (adjusted P value < 0.05, log2 fold change ≥ 1 for upregulated genes and adjusted P value < 0.05, log2 fold change ≤ 1 for downregulated genes).
  2. Box plots showing log2 fold change of genes significantly upregulated upon 15‐min treatment with 200 nM TPA in RPB1‐52R and RPB1‐25R cells (adjusted P value < 0.05, log2 fold change ≥ 1). P value = 0.02257 (Mann–Whitney test) Box limits are the first and third quartiles, the band inside the box is the median. The ends of the whiskers extend the box by 1.5 times the interquartile range. Two independent biological replicates were analyzed.
  3. Changes in RNA synthesis in response to 30 min of TPA treatment. MA plot showing RNA synthesis changes in TT‐seq datasets upon 30 min treatment with TPA (200 nM) in RPB1‐52R and RPB1‐25R cells. DMSO treatment of the respective cell line was used as a control and the data were normalized using library size normalization. 14,636 expressed genes annotated in RefSeq were analyzed. Differentially expressed genes are in red (adjusted P value < 0.05, log2 fold change ≥ 1 for upregulated genes and adjusted P value < 0.05, log2 fold change ≤ 1 for downregulated genes)
  4. Box plots showing log2 fold change in RNA synthesis of genes significantly upregulated upon 30‐min treatment with 200 nM TPA in RPB1‐52R and RPB1‐25R cells (adjusted P value < 0.05, log2 fold change ≥ 1). P value = 0.001749 (Mann–Whitney U‐test) Box limits are the first and third quartiles, the band inside the box is the median. The ends of the whiskers extend the box by 1.5 times the interquartile range. Two independent biological replicates were analyzed.