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YbeY is involved in the regulation of the T3SS. (A) Wild-type PA14, the ΔybeY mutant, and the complemented strain were grown in LB to an OD600 of 1. The relative mRNA levels of the T3SS genes were determined by RT-qPCR. Results represent means ± standard deviations (SD). (B) A549 cells were infected with the indicated strains at an MOI of 50 for 2 or 3 h. The relative cytotoxicity was determined by the LDH release assay. Results represent means ± SD. ***, P < 0.001 by Student's t test.
