FIG 3.

YbeY controls the expression of the T3SS and T6SS genes and biofilm formation through RsmY/Z-RsmA. (A) The indicated strains were grown in LB to an OD₆₀₀ of 1. The relative mRNA levels of the T3SS genes were determined by RT-qPCR. Results represent means ± SD. (B) A549 cells were infected with the indicated strains at an MOI of 50 for 2 h. The relative cytotoxicity was determined by the LDH release assay. (C) The relative mRNA levels of the T3SS genes were determined by RT-qPCR. Results represent means ± SD. (D) The relative bacterial cytotoxicity was determined by the LDH release assay. (E) The indicated strains were grown in LB to an OD₆₀₀ of 1. The relative mRNA levels of the T6SS genes were determined by RT-qPCR. (F) The indicated strains were grown in 96-well plates for 20 h. The wells were washed with PBS and stained with 1% crystal violet. The crystal violet was dissolved in ethanol and measured at a wavelength of 595 nm. Results represent means ± SD. **, P < 0.01; ***, P < 0.001; both by Student's t test.