FIG 5.

YbeY controls biofilm formation and the expression of T3SS and T6SS genes through RetS. (A) The indicated strains were grown in LB to an OD₆₀₀ of 1. The relative mRNA levels of the T3SS genes were determined by RT-qPCR. Results represent means ± SD. (B) A549 cells were infected with the indicated strains at an MOI of 50 for 3 h. The relative cytotoxicity was determined by the LDH release assay. (C) The indicated strains were grown in LB to an OD₆₀₀ of 1. The relative mRNA levels of the T6SS genes were determined by RT-qPCR. Results represent means ± SD. (D) The indicated strains were grown in 96-well plates for 20 h. The wells were washed with PBS and stained with 1% crystal violet. The crystal violet was dissolved in ethanol and measured at a wavelength of 595 nm. Results represent means ± SD. **, P < 0.01; ***, P < 0.001; both by Student's t test.