TABLE 1.

Bacterial strains, plasmids and primers used in this study^a

Strain, plasmid, or primer	Description or sequence (5′–3′)	Source (reference) or function
E. coli
DH5α	F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80dlacZΔM15	57
S17-1	Δ(lacZYA-argF)U169 hsdR17 (rK−mK+) λ-thi pro hsdR recA traC+	57
P. aeruginosa
PA14	Wild type	60
△ybeY	PA14 deleted of ybeY	30
△ybeY/att7::ybeY	△ybeY with ybeY inserted on chromosome with mini-Tn7T insertion; GENr	30
△ybeZ	PA14 deleted of ybeZ	30
△ybeZ/att7::ybeZ	△ybeZ with ybeZ inserted on chromosome with mini-Tn7T insertion; GENr	30
△rsmA	PA14 deleted of rsmA	This study
△ybeY△rsmA	PA14 deleted of ybeY and rsmA	This study
△retS	PA14 deleted of retS	This study
△ybeY△retS	PA14 deleted of ybeY and retS	This study
PA14/pUCP20	PA14 with empty plasmid pUCP20; CARr	This study
△ybeY/pUCP20	△ybeY with empty plasmid pUCP20; CARr	This study
△ybeY/pUCP20-rsmY	△ybeY with plasmid pUCP20-rsmY; CARr	This study
△ybeY/pUCP20-rsmZ	△ybeY with plasmid pUCP20-rsmZ; CARr	This study
Plasmids
pUCP20	Escherichia-Pseudomonas shuttle vector without lac promoter; AMPr	61
pEX18Tc	Gene replacement vector; TETr, oriT+, sacB+	61
pUC18T-mini-Tn7T-Gm	Mini-Tn7 base vector from insertion into chromosome attTn7 site; GENr	61
pDN19lacΩ	Promoterless lacZ fusion vector; SPTr, STRr, TETr	57
pEX18Tc-△rsmA	rsmA gene of PA14 deletion on pEX18Tc; TETr	This study
pEX18Tc-△retS	retS gene of PA14 deletion on pEX18Tc; TETr	This study
pUCP20-rsmY	Overpression of rsmY on pUCP20; CARr	This study
pUCP20-rsmZ	Overpression of rsmZ on pUCP20; CARr	This study
Primers
RsmA-L-F	CCGGAATTCGCACATCGACGACACCCAC	rsmA deletion
RsmA-L-R	TGCTCTAGACCCGACGAGTCAGAATCAGC	rsmA deletion
RsmA-R-F	TGCTCTAGAAGAAAGATCAAGAGCCAAACCA	rsmA deletion
RsmA-R-R	CCCAAGCTTCTTAGTCTTGCCCCCTATGGA	rsmA deletion
RsmA-T-F	AGGGTGAGTGACGCTGGCA	△rsmA screen
RsmA-T-R	GCCGCCTGAATCAACCTCTA	△rsmA screen
RetS-L-F	CCCAAGCTTGAAGCCAAGTGCGAGAACGT	retS deletion
RetS-L-R	TGCTCTAGAGAGCAGAAGCAGCAGGAAGC	retS deletion
RetS-R-F	TGCTCTAGAGGTGCTGATGGACTGCGAGA	retS deletion
RetS-T-F	CGGCCACTTGGCTATAATCC	△retS screen
RetS-T-R	CAGGACAGCACGAAGAAGGG	△retS screen
PA1805-RT-F	ATCAGTCTCAATGAAGTC	RT-PCR
PA1805-RT-R	CATGGATGGATCGAAATC	RT-PCR
RpsL-RT-F	GTAAGGTATGCCGTGTACG	RT-PCR
RpsL-RT-R	CACTACGCTGTGCTCTTG	RT-PCR
ExsA-RT-F	GCTATGTCGTAAGTACCA	RT-PCR
ExsA-RT-R	GAAGCCTTGTAGAAACTG	RT-PCR
ExsC-RT-F	CAGCTTCAACCGCCATTG	RT-PCR
ExsC-RT-R	CGCATACAACTGGACCTTG	RT-PCR
ExsD-RT-F	AGAGGTGCGGCAGATTCTCC	RT-PCR
ExsD-RT-R	ATCATCGACTGCGGCACG	RT-PCR
ExoU-RT-F	AACACATTAGCAGCGAGAT	RT-PCR
ExoU-RT-R	AGCAGCAACTCAGAGAAG	RT-PCR
PcrV-RT-F	CACGCTCTATGGCTATGC	RT-PCR
PcrV-RT-R	AAGGTATCCAGATTGCTCAG	RT-PCR
RsmA-RT-F	GAAGGAAGTCGCCGTACA	RT-PCR
RsmA-RT-R	TAATGGTTTGGCTCTTGATCTTT	RT-PCR
RsmY-RT-F	CCAAAGACAATACGGAAA	RT-PCR
RsmY-RT-R	GTTTTGCAGACCTCTATC	RT-PCR
RsmZ-RT-F	CAACCCCGAAGGTTC	RT-PCR
RsmZ-RT-R	CAGTCCCTCGTCATC	RT-PCR
GacA-RT-F	CCTGATGATCGCCAACTG	RT-PCR
GacA-RT-R	ATAGGTATTCACGGTCTTCG	RT-PCR
GacS-RT-F	GAGGAAATGCAGCACAAC	RT-PCR
GacS-RT-R	GTTCTGGATCTCGATGGT	RT-PCR
RetS-RT-F	GACTACGTGCAGACCATC	RT-PCR
RetS-RT-R	CTTGGAGATGTCGAGGAT	RT-PCR
LadS-RT-F	GATGCTGATCTACAACCT	RT-PCR
LadS-RT-R	GAAGCGATATAGAGGATGT	RT-PCR
HptB-RT-F	CATCTCGATGATCGTGTTC	RT-PCR
HptB-RT-R	GAAGGTATCCAGCAGGAC	RT-PCR
Hcp1-RT-F	AGGACCTGTCGTTCACCAA	RT-PCR
Hcp1-RT-R	ATAGTGCTTGCCGCTGGA	RT-PCR
VgrG1a-RT-F	GAGACCAGCTTCGACTTCATC	RT-PCR
VgrG1a-RT-R	CTTCTGCTCATGGCGGAAC	RT-PCR
Hcp3-RT-F	ACATCAAAGGCGACAGCC	RT-PCR
Hcp3-RT-R	GTTGCTGACGTCGTTGGT	RT-PCR
HsiB3-RT-F	ATCACCTACGACGTCGAGAT	RT-PCR
HsiB3-RT-R	GTCGATGTCGACGAAACGC	RT-PCR

^aGEN^r, gentamicin resistance; AMP^r, ampicillin resistance; TET^r, tetracycline resistance; CAR^r, carbenicilin resistance; STR^r, streptomycin resistance; SPT^r, spectinomycin resistance; KAN^r, kanamycin resistance. Enzyme cleavage sites are underlined.