FIG 6.

E. coli strain MKH13, which has deletions in all compatible solute transporters, was grown in M9G supplemented with 4% NaCl and functionally complemented with V. parahaemolyticus RIMD2210633 (A) bccT1 (pBAVP1456), bccT2 (pBAVP1723), bccT3 (pBAVP1905), or bccT4 (pBAVPA0356). Strains were grown for 24 h in the presence of 500 µM DMSP and the final optical density (OD₅₉₅) was compared to that of a strain harboring empty pBAD33. (B) E. coli MKH13 complemented with bccT1 was grown in the presence of 500 µM glycine betaine and compared to a strain harboring empty pBAD33. Means and standard errors of at three biological replicates are shown. Statistics were calculated using Student’s t test (***, P < 0.001; ****, P < 0.0001).