Skip to main content
. 2021 Apr 19;12:660841. doi: 10.3389/fphar.2021.660841

FIGURE 2.

FIGURE 2

Evaluation of in vitro uptake of Cy5-BDNF-AT and BDNF AT cationic liposomes and saline formulation in RT4-D6P2T rat schwannoma cells using confocal microscopy and AT hybridization assay. Confocal microscopic images of time dependent uptake of vehicle control and Cy5-BDNF-AT at 300 nM in RT4-D6P2T rat schwannoma cells demonstrating more rapid and robust transfection in the liposomal (AT-LIPO) vs. saline (AT-SALINE) group (bar = 500 μm). Histograms represent quantification of active BDNF-AT uptake by dose, time, and formulation in the RT4-D6P2T cell line relative to protein normalized control (NEG represents vehicle control). Note the significant dose dependent increase in uptake efficiency in the liposome relative to the saline and negative control groups (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; two-way ANOVA). Data are presented as mean ± SD (n = 4–6).