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Impact of pH variations assessed on the [35S]Cys-Met incorporation into phagosome. (A and C) Anaplasma cell-free phagosome. (B and D) E. chaffeensis cell-free phagosome. The first lane included axenic media with G6P, ATP, and chloramphenicol (CHL) to serve as the negative control. The pH of the negative-control medium was adjusted to 7. The incubation time and conditions were as in Fig. 2.
