TABLE 1.

E. chaffeensis and A. phagocytophilum 16S rRNA assessed by TaqMan probe-based real-time qRT-PCR assays for RNA^a

Incubation period (h)	CT (SD)	Fold reduction compared to 0 h	P valueb
E. chaffeensis
0	14.89 (0.24)	NAc	NA
2	15.96 (0.80)	2	0.0811
6	17.93 (0.26)	8	0.0056*
12	21.65 (0.23)	108	0.0016*
24	26.79 (0.31)	3,821	0.0001*
A. phagocytophilum
0	7.34 (0.81)	NA	NA
2	13.98 (2.22)	99	0.0329*
6	26.76 (1.01)	701,459	0.0459*
12	27.49 (1.50)	1,163,467	0.0344*
24	30.99 (0.07)	13,163,136	0.0170*

^a16S rRNA was assessed by TaqMan probe-based real-time qRT-PCR assays for RNA recovered from purified phagosomes following the different time points shown of incubation in the axenic media at pH 7.

^bAsterisks indicate significant fold change at different time points compared to the 0-h value.

^cNA, not applicable.