TABLE 1.
GMMA and OMV analytical characterization
| Antigen | Vi/OAga (wt/wt) ratio, % | Vi/proteina (wt/wt) ratio, % | OAg/proteina (wt/wt) ratio, % | Z avg diamb (nm) | Polydispersion indexb |
|---|---|---|---|---|---|
| ParA Vi+ GMMA | 9.6 | 4.2 | 43 | 83 | 0.14 |
| ParA Vi− GMMA | NA | NA | 47 | 72 | 0.13 |
| S. Typhi Vi+ OMV | 10.1 | 5.0 | 50 | 133 | 0.41 |
| S. Typhi Vi− OMV | NA | NA | 164 | 81 | 0.35 |
a
OAg content and Vi content were measured by HPAEC-PAD analysis and protein content by micro-BCA analysis, and reported ratios were calculated. NA, not applicable.
b
GMMA diameter and polydispersion index were calculated by DLS.