FIG 1.

Tsp is required for Lp to survive a thermal stress in water. The WT, tsp mutant (Δtsp), and complemented strain (Δtsp + ptsp) were suspended in Fraquil for 24 h and then subjected to thermal stress at 55°C. The survival of the strains was measured by CFU counts every 15 min from 0 to 60 min. The data shown represent the average of three independent biological replicates with standard deviation. A two-way analysis of variance (ANOVA) with a Tukey correction for multiple comparison was used to determine statistical difference (****, P < 0.0001).