FIG 4.

Tsp is expressed in the PE phase. Tsp was tagged with a hexahistidine tag and cloned in plasmid pXDC39 under its own promoter and transferred to the WT (WT + ptsp-His) and tsp mutant (Δtsp + ptsp-His) strains. The strains were grown to the E and PE phases in AYE broth, and expression of the Lpr17 was analyzed by Northern blotting (A), while the expression of the Tsp was analyzed by Western blotting (B). The strains with the empty vector (WT + empty vector; Δtsp + empty vector) served as negative controls. 5S rRNA and IcdH serve as the loading control for Northern blotting and Western blotting, respectively.