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. 2021 Apr 13;87(9):e00122-21. doi: 10.1128/AEM.00122-21

FIG 7.

FIG 7

Experimental setup of animal trials. (A) Animal trial with diets supplemented with cellulose or inulin with high or low calcium. After 14 days of acclimatization to the experimental diets, all rats receive a single gavage containing approximately 8 × 109 CFU of L. plantarum Lp900-R. Fecal pellets are collected daily up to 9 days postgavage, and the rats receive a second gavage containing approximately 6 × 109 CFU of L. plantarum Lp900-R to assess local persistence in different intestinal regions (ileum and colon) after 4, 5, or 24 h. (B) Animal trials with diets supplemented with high or low calcium. After 14 days of acclimatization to the experimental diets, half (n = 8) of each diet group receives a single probiotic gavage containing approximately 9 × 109 CFU of L. plantarum Lp900-R or a single synbiotic gavage containing 9 × 109 CFU of L. plantarum Lp900-R suspended in 1 ml of inulin solution (27.5% [wt/vol]). Fecal pellets are collected daily up to 9 days postgavage, and then the rats receive a second gavage (analogous to the initial gavage) containing approximately 6 × 109 CFU of L. plantarum Lp900-R to assess local persistence in different intestinal regions (ileum and colon) after 4, 5, or 24 h.