TABLE 1.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant feature(s) or sequence (5′–3′) | Source or reference |
|---|---|---|
| Strains | ||
| L. plantarum WCFS1 | Single-colony isolate of L. plantarum NCIMB8826 | 68 |
| L. plantarum Lp900 | Harbors six plasmids, one of which encodes an “FOS operon,” including a β-fructosidase | Fuhren et al., unpublished |
| L. plantarum Lp900-R | Rifampin-resistant derivative of Lp900 | This study |
| L. plantarum NC8 | Plasmid-free strain of L. plantarum | 69 |
| L. lactis NZ9000 | MG1263 derivative harboring nisRK integrated in its genome | 70 |
| L. lactis MG1363 | NCDO712-derived strain that lacks plasmids and prophages | 71 |
| Plasmids | ||
| pNZ278 | Cmr; containing gusA under control of the bacteriophage ϕSK11G promoter | 33 |
| pNZ278-fosE | Cmr; pNZ278 derivative in which the gusA coding region is replaced by L. plantarum Lp900 fosE | This study |
| Primers | ||
| S1 | GAGGACGGTTCTAAGTCGTTATCCTGGTGAAATGATTCCA | This study |
| S2 | AACATGCTGAAGAGCATCTCATCGCTGGTAAAACGCTCAC | This study |
| S3 | GTGAGCGTTTTACCAGCGATGAGATGCTCTTCAGCATGTT | This study |
| S4 | TGGAATCATTTCACCAGGATAACGACTTAGAACCGTCCTC | This study |
| S5 | CCTTAACGCAGTTGCTGTTA | This study |
| S6 | GCTGTTCACTGAAGATTGCC | This study |