FIG 4.

Reverse engineering of the key mutations found in ALE-derived strains. Reverse engineering of the key mutations found in the ALE-derived P. putida S12 strains successfully restored the solvent tolerance phenotype in the plasmid-cured strain S12-10. Different colors indicate the control strain S12-10 and the reverse engineering (RE) strains. This experiment was performed with three biological replicates, and error bars indicate standard deviations. The y axis may be different for the panels shown.