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. 2021 Apr 13;87(9):e00091-21. doi: 10.1128/AEM.00091-21

FIG 2.

FIG 2

(A to F) Atomized-oil assays to demonstrate the production of surfactants. (A to D) Wild-type colonies of PFF1, PFF2, PFF3, and PFF4, respectively, exhibiting a halo indicative of surfactant production. (E) Tween 20. (F) E. coli DH5α. (G to L) Drop collapse assays to demonstrate the production of surfactants. (G to J) Culture supernatants of wild-type PFF1, PFF2, PFF3, and PFF4, respectively, collapsed into oil, indicative of surfactant production. (K) Collapsed drop containing Tween 20. (J) Noncollapsed drop of E. coli culture supernatant (arrow).