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Knockout mutants show no sign of surfactant production. MS/MS spectra of extracts of PFF1::ezTn5-viscB, PFF2::ezTn5-viscB, PFF3::ezTn5-massB, and PFF4::ezTn5-massB (A to D, respectively), are shown. None of the random knockout mutants produced detectable surfactant peaks at the corresponding wild-type m/z values. Spectra were normalized against the maximal intensity.
