TABLE 1.
Fold change in expression of selected genes from the B. diazoefficiens T3SS in starvation cultures under genistein-induced relative to noninduced conditionsa
| Gene | Fold change in expression vs noninducedb |
|
|---|---|---|
| Wild type | rsh mutant | |
| ttsI | 29.66 ± 3.72 A | 2.83 ± 1.26 B |
| rhcJ | 42.60 ± 22.00 A | −1.45 ± 0.02 B |
a
Induction was performed with 2 μM genistein in starvation medium. The housekeeping gene sigA was used as an internal control.
b
Values are the average ± SE from three independent experiments. Statistical analysis was performed by ANOVA. Values followed by different letters in the same row are significantly different (P < 0.0001).