TABLE 4.
Primers used in this study
| Name | Sequence | Source or reference |
|---|---|---|
| rsh mutant construction and complementation | ||
| rsh1 Fw | TACAATCCCAACACCAACGA | This study |
| rsh2 Rv | ACGCGCTCCTCCTTGTAGT | This study |
| rsh-compl-Fw | AAAAATCTAGAATACCGAAGTTGCCGTTGAG | This study |
| rsh-compl-Rv | AAAAACTGCAGCACAAATTCGAAGGA | This study |
| Km F | CATCGGGCTTCCCATACA | 73 |
| Km R | TGCCATTCTCACCGGATT | 73 |
| Plant defense | ||
| PR1_Fw | ATGTTGCCTACGCTCAAGATTCAGCA | This study |
| PR1_Rv | AGCAACCGTATCATCCCAAGCCAAACT | This study |
| T3SS | ||
| blr1813_Fw | GACGGCTTCGCGTGTTTC | This study |
| blr1813_Rv | GGACAGCATCAACGCCCT | This study |
| bll1843_Fw | ACCTCACGCCGAGCAATC | This study |
| bll1843_Rv | CGGGCCATTTTGCGAAGG | This study |
| Regulation of nodulation | ||
| RIC1_Fw | GTCGGCAGTAGTAGTTGTATGG | This study |
| RIC1_Rv | AGCCTCCTCACTGAACTTGT | This study |
| NIC1_Fw | CTAAGCCCTGGAGGACCTGA | This study |
| NIC1_Rv | CAGCATACGTGGTAATTGCCTG | This study |
| RIC2_Fw | TGTGGGTGATGTAGGGTGGAT | This study |
| RIC2_Rv | CCTGGTTCGTCTTCATTGATCTCC | This study |
| Soybean reference gene | ||
| Ef1-α_Fw | GGTCATTGGTCATGTCGACTCTGG | 74 |
| Ef1-α_Rv | GCACCCAGGCATACTTGAATGACC | 74 |
| B. diazoefficiens reference gene | ||
| sigA-1533F | CTGATCCAGGAAGGCAACATC | 75 |
| sigA-1617R | TGGCGTAGGTCGAGAACTTGT | 75 |