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. 2021 Mar 9;30(3-4):265–276. doi: 10.1093/hmg/ddab040

Figure 3.

Figure 3

Proinflammatory cytokines production by WS PBMCs. (A) Schematic heat map representation of Bio-Plex Pro Human Th17 cytokine panel. The cytokine profile was analyzed in supernatants from PBMCs from healthy donors (Ctrl) and from proband’s PBMCs (WS). The contents of selected cytokines were also analyzed by single-cytokine ELISA test and data were reported in (B). (C) Real-time PCR analysis of TNF-α, IL-1β, IL-6 transcripts in freshly purified PBMCs from healthy controls (Ctrl) or the WS patient. Data were normalized to the expression of ACTB (encoding β-actin) and presented relative to results in healthy controls untreated cells (dotted line, 1-fold). In (B) and (C) data were analyzed by two-tailed Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. (D) Schematic heat map representation of Cytokine 25-Plex Human ProcartaPlex™ Panel 1B. The cytokine profile was analyzed in sera from healthy donors (Ctrl), type 1 diabetes subjects (T1D) and the WS proband.