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. 2021 Mar 9;30(3-4):265–276. doi: 10.1093/hmg/ddab040

Figure 4.

Figure 4

Production of proinflammatory cytokines after silencing of the WFS1 gene in PBMCs from healthy donors. (A) The mRNA level of WFS1 after transfection with WFS1 siRNA was detected by real-time PCR in PBMCs of three healthy donors. Control cells were treated with vehicle alone (DOTAP). Data were normalized to the expression of ACTB (encoding β-actin) and presented relative to results in untreated cells (dotted line, 1-fold). One experiment is shown representative of three. Data were analyzed by one-way ANOVA followed by post hoc Bonferroni’s test. (B) Schematic heat map representation of Bio-Plex Pro Human Th17 cytokine panel. The cytokine profile was analyzed in supernatants from PBMCs of healthy donors untransfected or transfected with siWFS1 or a scramble siRNA. Vehicle alone (DOTAP) was used as a control. Individual expression levels of selected cytokines were reported in (C). Data were analyzed by one-way ANOVA followed by post hoc Bonferroni’s test. **P < 0.01; ***P < 0.001; ****P < 0.0001.