TABLE 2.
Changes to body temperature as assessed by behavioral fever. Time spent (in seconds) by individual fish in three different temperature chambers (23, 29, and 37°C). Data is shown as mean ± S.D. of 24 individuals, analyzed by Two-Way ANOVA followed by Tukey’s multiple comparison test (#) denotes p < 0.0001 compared to the normal control (*) denotes p < 0.0001 compared to disease control, and (a) denotes p < 0.0001 when compared to 37°C.
| Study groups | 4th day time point | 7th day time point | ||||
|---|---|---|---|---|---|---|
| 23°C | 29°C | 37°C | 23°C | 29°C | 37°C | |
| Normal control | 35.58 ± 3.0 | 129.63 ± 1.4 | 14.92 ± 2.7 | 24.13 ± 0.7 | 130.00 ± 0.8 | 25.88 ± 0.7 |
| HZF | 37.79 ± 1.5 | 134.00 ± 1.5 | 8.21 ± 2.0 | 25.88 ± 1.3 | 135.75 ± 1.5 | 18.38 ± 1.3 |
| HZF-SARS-CoV-2 | 15.96 ± 1.6# | 7.04 ± 1.3# | 157.08 ± 1.6# | 2.38 ± 1.1# | 4.75 ± 1.3# | 172.88 ± 1.3# |
| Dexamethasone | 21.13 ± 2.3* | 30.46 ± 2.3* | 128.29 ± 1.9* | 45.54 ± 1.6* | 57.54 ±2.0* | 76.92 ± 2.1* |
| GG-6 µg/kg | 1.21 ± 2.1* | 36.17 ± 2.6* | 142.63 ± 2.3* | 0.00* | 11.92 ± 1.9* | 168.08 ±1.9* |
| GG-28 μg/kg | 0.58 ± 1.2* | 48.46 ± 1.9* | 130.96 ± 2.1* | 14.25 ± 1.8* | 25.13 ± 1.8* | 140.63 ± 1.9* |
| GG-142 μg/kg | 20.17 ± 4.5* | 69.92 ± 3.5* | 89.92 ± 4.2* | 0.00* | 123.96 ±2.0*/a | 56.04 ± 2.0* |