. 2021 Apr 3;24(5):102391. doi: 10.1016/j.isci.2021.102391

Table 1.

Mutation of Mycoplasma mycoides JCVI-Syn3B, and Whole Genome Sequence (WGS) analysis identified the mutations (bold and underlined)

Syn3B Strains	Intergenic mutation^a	Mutation positions^b	Genotype change	Amino acid substitution	Gene	Gene annotation
Parent^∗
P026	9	547	GCA → ACA	Ala → Thr	dnaA⁺	Chromosomal replication initiator protein DnaA
P026	9	274928	TAC → TAT	Tyr → Tyr	fakA^c⁺	Dihydroxyacetone kinase
Ksg^R
PK07-L1	8	479174	CCT → ACT	Pro → Thr	rpoC⁺	DNA-directed RNA polymerase subunit beta
PK07-L1	8	3322	GGA → GAA	Gly → Glu	ksgA⁺	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase
PK07-L2	8	479174	CCT → ACT	Pro → Thr	rpoC⁺
PK07-L2	8	3322	GGA → GAA	Gly → Glu	ksgA⁺
Strep^R
PS04-L1	4	101452	AAA → AGA	Lys → Arg	rpsL⁺	30S ribosomal protein S12
PS04-L1	4	33,652	GGA → AGA	Gly → Arg	CDS_6⁺	Unknown
PS04-L2	3	101452	AAA → AGA	Lys → Arg	rpsL⁺
		147803	AGC → ATC	Ser → Ile;	rpsD⁺	30S ribosomal protein S4
		33,652	GGA → AGA	Gly → Arg	CDS 6⁺
Cip^R
PC06-L1	4	7735	GAA → AAA	Glu→ Lys	gyrA⁺	DNA gyrase subunit A
PC06-L1	4	305613	GAT → AAT	Asp→ Asn	parC⁺	DNA topoisomerase 4 subunit A
PC06-L2	4	7735	GAA → AAA	Glu→ Lys	gyrA⁺
		305613	GAT → AAT	Asp→ Asn	parC⁺
		304707	GAT → GCT	Asp→ Ala	parE⁺	DNA topoisomerase 4 subunit B
		33,652	GGA → AGA	Gly → Arg	CDS_6⁺
Strep^R-Cip^R
PSC09-L1	4	7725	AGT → AGG	Ser→ Arg	gyrA⁺
		101452	AAA → AGA	Lys → Arg	rpsL⁺
		33,652	GGA → AGA	Gly → Arg	CDS_6⁺
		305602	AGT → ATT	Ser→ Ile	parC⁺
PSC09-L2	4	7725	AGT → AGG	Ser→ Arg	gyrA⁺
		101452	AAA → AGA	Lys → Arg	rpsL⁺
		33,652	GGA → AGA	Gly → Arg	CDS_6⁺
		305602	AGT → ATT	Ser→ Ile	parC⁺

All antibiotic-resistant mutant strains were named based on the number of passes (P) in a specific antibiotic (K: Ksg, S: Strep, C: Cip, SC: Strep-Cip). All antibiotic-resistant mutants were selected from two separate evolutionary lineages, and named L1 for lineage 1 or L2 for linage 2.

∗Parent: High growth rate mutant parent to all of the strains in the table.

⁺Mutated genes are likely to be functional, although the mutated genes' functionality has not been tested in this study.

Mutation in the non-coding DNA sequences located between genes.

Genomic position numberings correspond to Mycoplasma mycoides JCVI-Syn3B and P026 (CP053944).

Synonymous mutation: mutation does not change in the encoded amino acid sequence.