Table 2.

Syn3B contains genes previously shown to be related to E.coli persistence and tolerance

Gene	Protein/RNA	Major function	REF
dnaK, dnaJ, clpB	Chaperon	Global regulator	(Wu et al., 2015; Hansen et al., 2008)
phoU	Phosphate-specific transport system accessory protein	Global regulator	(Li and Zhang, 2007)
xseB	Exodeoxyribonuclease	DNA mismatch repair and recombination	(Cui et al., 2018)
uvrA, uvrB	Endonuclease	SOS response	(Wu et al., 2015; Cui et al., 2018; Keren et al., 2004)
relA	GTP pyrophosphokinase	Stringent response	(Wu et al., 2015)
lon	Protease	Protease	(Wu et al., 2015)
glyA	Serine hydroxymethyltransferase	Metabolism	(Cui et al., 2018)
atpA, atpC, atpF	ATP synthase	ATP synthesis	(Girgis et al., 2012; Lobritz et al., 2015; Kiss, 2000)
galU	UTP–glucose-1-phosphate uridylyltransferase	Lipopolysaccharide precursor synthesis	(Girgis et al., 2012)
trmE; efp; ybeY	tRNA modification GTPase; Elongation factor P; Endoribonuclease	Translation	(Cui et al., 2018)
ssrA; smpB	Transfer messenger RNA (tmRNA); Small Protein B	Trans-translation	(Wu et al., 2015; Yamasaki et al., 2020)