Figure 3.

Influence of single point mutation of the 12-bp symmetrical region in CRE_Tre for CcpA protein binding

(A) Single point mutation of the 12-bp symmetrical region of CRE_Tre.

(B) EMSA of CcpA protein for 12 mutants (5′ biotin), 12 mutants (T1, T2, T3, T4, T5, T6, T7, T8, T9, T10, T11, and T12) derived from CRE_Tre, with concentrations of 0.9 μM–2.0 μM of CcpA protein.

(C) The ratio of protein-bound probe/total probe for 12 mutants that derived from CRE_Tre at the 0.9 μM CcpA protein was shown. Statistical significance was determined by Student's t-test (∗P ≤ 0.05; ∗∗P ≤ 0.01; ∗∗∗P ≤ 0.001).