TABLE 2.
Clinical and demographic characteristics of patients included in the second cohort for qRT-PCR validation
| Characteristic | Preterm labor and delivery without intra-amniotic inflammation (n = 16) | Preterm labor and delivery with sterile intra-amniotic inflammation (n = 19) | Preterm labor and delivery with intra-amniotic infection (n = 19) | P value |
|---|---|---|---|---|
| Maternal age (median [IQR] [yrs])a | 23 (19.8–23.8) | 26 (22.5–28.5) | 23 (20–26) | 0.07 |
| Body mass index (median [IQR] [kg/m2])a | 21.8 (20.1–27)c | 29.8 (25.4–33.8)d | 26.9 (22.5–34)d | 0.03 |
| Primiparityb (% [no. of patients/total no. of patients]) | 31.3 (5/16) | 21.1 (4/19) | 21.1 (4/19) | 0.8 |
| Race/ethnicityb (% [no. of patients/total no. of patients]) | 0.4 | |||
| African-American | 87.5 (14/16) | 89.5 (17/19) | 94.7 (18/19) | |
| White | 12.5 (2/16) | 10.5 (2/19) | 0 (0/19) | |
| Other | 0 (0/16) | 0 (0/19) | 5.3 (1/19) | |
| Gestational age at amniocentesis (median [IQR] [wks])a | 31.8 (29.9–33.6) | 26.4 (23.9–29.7) | 26.7 (23.7–31.2) | 0.02 |
| IL-6 (median [IQR] [ng/mL])a | 0.2 (0.2–0.5) | 11.2 (5.7–33.2) | 121.8 (59.3–153.9) | <0.001 |
| Amniotic fluid glucose (median [IQR] [mg/dL])a | 24 (18–30)c | 21 (18–26)d | 5 (1–17)e | 0.002 |
| Amniotic fluid WBC (median [IQR] [cells/mm3])a | 0 (0–0)c | 3 (1–14)d | 292 (43.3–467.5)e | <0.001 |
| Gestational age at delivery (median [IQR] [wks])a | 34.3 (33.3–34.9) | 26.7 (24.5–30.5) | 26.7 (23.8–31.2) | <0.001 |
| Cesarean sectionb (% [no. of patients/total no. of patients]) | 18.8 (3/16) | 26.3 (5/19) | 26.3 (5/19) | 0.9 |
| Birth weight (median [IQR] [g])a | 2,230 (1,807–2,575) | 917 (635–1,545) | 1,040 (587.5–1,447.5) | <0.001 |
| Acute maternal inflammatory responseb (% [no. of patients/total no. of patients]) | ||||
| Stage 1 (early acute subchorionitis or chorionitis) | 12.5 (2/16) | 21.1 (4/19) | 10.5 (2/19) | 0.7 |
| Stage 2 (acute chorioamnionitis) | 18.8 (3/16) | 10.5 (2/19) | 21.1 (4/19) | 0.7 |
| Stage 3 (necrotizing chorioamnionitis) | 0 (0/16) | 26.3 (5/19) | 63.2 (12/19) | <0.001 |
| Acute fetal inflammatory responseb (% [no. of patients/total no. of patients]) | ||||
| Stage 1 (chorionic vasculitis or umbilical phlebitis) | 6.3 (1/16) | 31.6 (6/19) | 26.3 (5/19) | 0.2 |
| Stage 2 (umbilical arteritis) | 6.3 (1/16) | 5.3 (1/19) | 57.9 (11/19) | <0.001 |
| Stage 3 (necrotizing funisitis) | 0 (0/16) | 0 (0/19) | 0 (0/19) | 1.0 |
a
Kruskal-Wallis/ANOVA test with correction for multiple comparisons.
b
Fisher’s exact test.
c
One missing datum.
d
Two missing data.
e
Three missing data.
f
IQR, interquartile range; yrs, years; wks, weeks; WBC, white blood cells.