TABLE 3.
Susceptibility of S. aureus to antibiotics and palmitoleic acid in the presence or absence of reserpine at 50 μg/ml
| S. aureus strain or mutantb,c | MIC (μg/ml) of:a |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TET | MINO | FOS | TUNI | PAL | TET | MINO | FOS | TUNI | PAL | |
| Without reserpine | With reserpine at 50 μg/ml | |||||||||
| RN6390 | 0.5 | 0.5 | 8 | 8 | 125 | 0.25 | 0.25 | 4 | 4 | 62.5 |
| QT7 (tet38::cat) | 0.25 | 0.5 | 4 | 4 | 62.5 | 0.25 | 0.5 | 4 | 4 | 62.5 |
| QT7 (pLI50) | 0.25 | 0.5 | 4 | 4 | 62.5 | 0.25 | 0.5 | 4 | 4 | 62.5 |
| QT7 (pLI50-tet38) | 4 | 2 | 16 | 16 | 500 | 1 | 0.5 | 4 | 4 | 125 |
| QT7 (pLI50-tet38-R106C) | 0.25 | 0.5 | 4 | 16 | 500 | 0.25 | 0.5 | 4 | 4 | 250 |
| QT7 (pLI50-tet38-G151C) | 0.5 | 0.5 | 4 | 4 | 250 | 0.25 | 0.5 | 4 | 4 | 125 |
| QT7 (pLI50-tet38-ΔL1) | 0.5 | 1 | 4 | 8 | 250 | 0.25 | 0.5 | 4 | 4 | 125 |
| QT7 (pLI50-tet38-ΔL7) | 1 | 1 | 8 | 16 | 500 | 0.5 | 0.5 | 4 | 8 | 250 |
a
TET, tetracycline; MINO, minocycline; FOS, fosfomycin; TUNI, tunicamycin; PAL, palmitoleic acid.
b
All strains harboring plasmid pLI50 were grown in the presence of 20 μg/ml chloramphenicol at 37°C.
c
QT7, tet38 mutant.