TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Relevant characteristic or description | Source or reference |
|---|---|---|
| JS0135 | G. parasuis isolate, serotype 4 | China |
| ΔnanH::kan | JS0135 with sialidase deficient | This study |
| ΔnanH::kan + pNanH | JS0135 ΔnanH::kan carrying expression plasmid pNanH | This study |
| pK18mobsacB | Suicide vector, kanR | (32) |
| pK18-nanH | pK18mobsacB, ΔnanH::kanR | This study |
| pSHG3 | Shuttle vector, gmR | (31) |
| pNanH | pSHG3 carrying nanH gene | This study |
| pET-25b | Expression vector, ampR | Novagen |
| pET-NanH | pET-25b expressing sialidase, ampR | This study |
| p3XFlagCMV-14 | CMV promoter, 3× FLAG, ampR | Novagen |
| pSiglec-5-Flag | p3XFlagCMV-14 carrying Siglec-5 gene | This study |