Figure S3.

BFAR is critical for Th9 differentiation without affecting cellular proliferation and apoptosis. (A and B) Flow cytometric analysis of the frequencies of IL-9–producing Th9 cells in WT, BFAR-deficient naive (A), and WT and BFAR-deficient naive or memory CD4⁺ T cells (B) cultured with the indicated concentrations of IL-4 and/or TGFβ1 for 3 d. Med, medium. (C) qPCR analysis of the Ifng, Il4, Il17a, Foxp3, Il9, Il1a, Ccl17, and Ccl20 mRNA expression of the naive CD4⁺ T cells cultured under distinct differentiation conditions as indicated for 3 d. (D–G) Flow cytometric analysis of the proliferation (D and E) and apoptosis (F and G) in WT and BFAR-deficient naive CD4⁺ T cells or cultured under Th0 or Th9 conditions for 3 d. The proliferation ratio was assessed as CFSE dilution by FACS. Data are presented as representative plots (D and F) and summary graphs (E and G). Each panel is representative of two independent experiments. Student’s t test was used. Bars, mean; error bars, SEM; **, P < 0.01; ns, not significant.