TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference(s) or source |
|---|---|---|
| Strains | ||
| S. aureus PJI | PJI isolate | 30 |
| S. aureus USA300 | Reference MRSAa wild-type strain | |
| S. aureus BB1929 | agr mutant of USA300; does not produce PSM | Horswill Lab collection |
| E. faecalis PJI | PJI isolate | 30 |
| C. striatum PJI | PJI isolate | 30 |
| P. acnes | ATCC 11828 | ATCC |
| P. aeruginosa | ||
| JDS25 | PAO1C (ATCC 15692) ΔpilA; markerless | 12, 50, 51 |
| CSM10 | JDS25 ΔfliC; markerless | This study |
| CSM12 | CSM10 ΔrhlAB; markerless | This study |
| CSM13 | CSM12::miniTn7 gfp2; Cmr Gmr | This study |
| Plasmids | ||
| pEX18Gm | Allelic replacement vector; Gmr | 52 |
| pRK600 | Mobilization plasmid; Cmr | 53 |
| pUX-BF13 | Conjugation helper plasmid; Apr | 54 |
| pBK-miniTn7-gfp2 | miniTn7 gfp2; Cmr Apr Gmr | 55 |
| pCSM103 | fliC allelic replacement vector in pEX18Gm; Gmr | This study |
| pAEM1 | rhlAB allelic replacement vector in pEX18Gm; Gmr | This study |
a
MRSA, methicillin-resistant S. aureus.