. 2021 Mar 26;87(8):e02889-20. doi: 10.1128/AEM.02889-20

TABLE 4.

Defensive effects of the hlyR4-deficient Hfx. mediterranei mutants against halocin-containing supernatants

Indicator plate (Hfx. mediterranei strain)^a	Inhibition activity at indicated dilution of halocin-containing supernatant^b
Indicator plate (Hfx. mediterranei strain)^a	1	1/2	1/4	1/8	1/16	1/32
EPS	●	●	●	○	○	○
EPSH	●	●	●	○	○	○
EPSHR	●	●	●	●	●	○
EPSHR-R4	●	●	○	○	○	○
EPSR	●	●	●	●	●	○
EPSR-R4	●	●	●	○	○	○

Strain EPS, Hfx. mediterranei strain DF50 ΔEPS; strain EPSH, Hfx. mediterranei strain DF50 ΔEPS ΔhalH4; strain EPSHR, Hfx. mediterranei strain DF50 ΔEPS ΔhalH4 ΔhlyR4; strain EPSHR-R4, Hfx. mediterranei strain DF50 ΔEPS ΔhalH4 ΔhlyR4::hlyR4; strain EPSR, Hfx. mediterranei strain DF50 ΔEPS ΔhlyR4; strain EPSR-R4, Hfx. mediterranei strain DF50 ΔEPS ΔhlyR4::hlyR4.

The dilution shown as 1 represents the 10-fold-concentrated halocin-containing supernatants produced by Haloferax sp. Q22 at late stationary phase, used for dilution. 1/2, 1/4, 1/8, 1/16, and 1/32 represent 2-, 4-, 8-, 16-, and 32-fold dilutions, respectively. Filled circle, significant inhibition activity; empty circle, no inhibition activity. These results were obtained through three biological replicates.