TABLE 5.
Genetically engineered strains and plasmids used in this study
| Strain (abbreviation) or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Haloferax mediterranei ATCC 33500 | Wild-type haloarchaeal strain | CGMCCa |
| Hfx. mediterranei strain DF50 (DF50) | pyrF gene deletion mutant of Hfx. mediterranei ATCC 33500 | 41 |
| Hfx. mediterranei strain DF50 ΔEPS (EPS) | eps gene deletion mutant of Hfx. mediterranei DF50 | 26 |
| Hfx. mediterranei strain DF50 ΔEPS ΔhalH4 (EPSH) | halH4 deletion mutant of Hfx. mediterranei strain EPS | 9 |
| Hfx. mediterranei strain DF50 ΔEPS ΔhlyR4 (EPSR) | hlyR4 deletion mutant of Hfx. mediterranei strain EPS | This study |
| Hfx. mediterranei strain DF50 ΔEPS ΔhlyR4::hlyR4 (EPSR-R4) | Strain EPSR harboring a recombinant plasmid, pWR4; hlyR4+ pyrF+ | This study |
| Hfx. mediterranei strain DF50 ΔEPS ΔhalH4 ΔhlyR4 (EPSHR) | hlyR4 and halH4 double deletion mutant of Hfx. mediterranei strain EPS | This study |
| Hfx. mediterranei strain DF50 ΔEPS ΔhalH4 ΔhlyR4::hlyR4 (EPSHR-R4) | Strain EPSHR harboring a recombinant plasmid, pWR4; hlyR4+ pyrF+ | This study |
| Hfx. mediterranei strain DF50 ΔEPS ΔhalH4::halH4 (EPSH-H4) | Strain EPSH harboring a recombinant plasmid, pWH4; halH4+ pyrF+ | 9 |
| Haloarcula hispanica ATCC 33960 | Wild-type haloarchaeal strain | CGMCC |
| Haloarcula hispanica strain DF60 (DF60) | ΔpyrF strain of Haloarcula hispanica ATCC 33960 | 45 |
| Haloarcula hispanica strain DF60-R4 (DF60-R4) | Haloarcula hispanica strain DF60 harboring a recombinant plasmid, pWR4; hlyR4+ pyrF+ | This study |
| Escherichia coli JM109 | Widely used host strain for molecular cloning; recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 | Novagen |
| E. coli JM110 | dam- and dcm-negative strain of E. coli JM109 | TaKaRa |
| Plasmids | ||
| pMD-18T | 2.7-kb cloning T-vector; Ampr | TaKaRa |
| pHFX | 4.0 kb, original plasmid for gene knockout lacking the origin of replication in haloarchaea; Ampr | 41 |
| pHFX-UDR4 | The upstream (509-bp) and downstream (541-bp) fragments of hlyR4 were linked and inserted into plasmid pHFX at the multiple cloning sites for gene knockout of hlyR4 | This study |
| pWL502 | 7.9-kb shuttle vector with pyrF gene; Ampr | 41 |
| pWR4 | 9.5-kb derivative of pWL502 containing hlyR4 and its native promoter | This study |
a
CGMCC, China General Microbiological Culture Collection Center.