TABLE 6.
Oligonucleotides used in this study
| Name | Sequence (5′–3′)a | Description |
|---|---|---|
| EPS16SF | GCGGACGATAACCTCGGGAAACTG | Internal reference for RT-PCR of halH4 and hlyR4 (393 bp) |
| EPS16SR | GCGGCTTTAGGCCCAATAATATCG | |
| H4F | ATTACACCGACTTTGCGCTC | For detection of halH4 (469 bp) |
| H4R | GCAACGTACACCATCTCGTC | |
| UPR4F1 | CGCAAGCTTCCGCGCGACCCTCGACGGCA | For amplification of upstream fragment of hlyR4 (509 bp) |
| UPR4R1 | CGCTTTCTTGGGGAGAGGGGCAAACATGACTCAGTGCACTACGATATTA | |
| DWR4F2 | CTGAGTCATGTTTGCCCCTCTCCCCAAGAAAGCGCGCTAGCCGTCTTCC | For amplification of downstream fragment of hlyR4 (541 bp) |
| DWR4R2 | CGCGGTACCGTCAGACCTCTCTCGGCACC | |
| R4DPF | CTACGCTGCAGTCAAGTTCC | For detection of hlyR4 (567 bp) |
| R4DPR | TTCATCGTCGAGGAGTAGCC | |
| 33500HlyF | GCGGGTACCGCAGACCGACGGAGCCGACG | For amplification of complete hlyR4 with its native promoter (1,700 bp) |
| 33500HlyR | GCGGGATCCTTATTCCTTTTCCGTGATGG |
a
Restriction sites HindIII (AAGCTT), KpnI (GGTACC), and BamHI (GGATCC) are in italics.